Margaret Bennett is Annie's biological mother.
As Agent Ness of the FBI revealed in the final chapters of the novelisation, Margaret, along with his husband David Bennett, were struggling artists from Iowa who went to New York City in order to study art at Cooper Union. With no known immediate relatives to call for, David left his daughter Annie at the orphanage as his wife contracted influenza, hoping he and Margaret would come back as soon as Annie's mother gets well. Sadly Margaret succumbed to her illness on January 13, 1922; David Bennett had also died as well, having contracted the disease from his wife. Margaret was described as "a pretty, sweet-faced blonde."
Later renditions of the musical adaptations would change their backstory somewhat, with David and Margaret having died in a fire - this alternate backstory would be used in the 1982 and 1999 renditions.